KMID : 0360220090500101563
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Journal of the Korean Ophthalmological Society 2009 Volume.50 No. 10 p.1563 ~ p.1568
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Effect of High Glucose on the Oxidative Stress in Trabecular Meshwork Cells
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Kim Jae-Woo
Bae Chang-Beum Lee Jeong-Hun
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Abstract
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Purpose: To investigate the effect of high glucose (HG) on the oxidative stress in cultured human trabecular meshwork cells (HTMC).
Methods: Primarily cultured HTMC were exposed to low glucose (5 mM) and HG (25 mM) for 7 days. Additionally, 1 mM Larginine, 5 mM DAHP, 10 ¥ìg/ml insulin, 100 ¥ìM L-ascorbic acid, 10, and 100 ¥ìM sepiapterin were co-exposed. The cellular survival and nitric oxide (NO) production were assessed by MTT assay and Griess assay, respectively. Superoxide production was measured by modified cytochrome c assay.
Results: HG did not affect the survival of cultured HTMC significantly. HG decreased NO production. Co-exposed DAHP decreased but DAHP and insulin increased NO production. In addition, HG increased superoxide production, which was decreased by insulin, L-ascorbic acid, and sepiapterin.
Conclusions: HG decreased NO production accompanied with increased superoxide production in HTMC. Thus HG induces oxidative stress in HTMC and may cause cellular dysfunction and damage of the trabecular meshwork.
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KEYWORD
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Glucose, Nitric oxide, Oxidative stress, Superoxide, Trabecular meshwork cells
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